Structure and Protective Capacity of Tetanus and Diphtheria Antibodies Produced During Human Pregnancy and Transferred to New-Born

Authors

  • MARCELA F. PASETTI,

    Corresponding author
    1. IDEHU, Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, (1113) Buenos Aires, Argentina
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  • JOSÉ DOKMETJIAN,

    1. IDEHU, Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, (1113) Buenos Aires, Argentina
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  • PATRICIA V. ERIKSSON,

    1. IDEHU, Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, (1113) Buenos Aires, Argentina
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  • Dr. MARCELA A. MANGHI,

    1. IDEHU, Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, (1113) Buenos Aires, Argentina
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  • MARÍA LUISA BRERO,

    1. Departamento de Contralor, Instituto Nacional de Microbiología “Dr. Carlos Malbrán,” (1281) Buenos Aires, Argentina
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  • FERNANDO FERRERO

    1. Hospital General de Niños “Pedro de Elizalde,” (1270) Buenos Aires, Argentina
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IDEHU-Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (1113 Buenos Aires, Argentina.

Abstract

PROBLEM: The structure and protective activity of antibodies against tetanus (anti-T) and diphtheria (anti-D), produced during human pregnancy and transferred to new-born, was studied.

METHOD: Antibody levels were measured by ELISA in non-pregnant women (control group), primiparae, and multiparae, and in their children. The proportion of symmetric and asymmetric IgG molecules was determined and their respective protective capacity evaluated.

RESULTS: The quantity of asymmetric anti-T and anti-D antibodies in mothers at the time of delivery was roughly four- and three-fold that of the control group, respectively, dropping significantly 1 month later. A similar proportion of these antibodies was observed in the new-born. The lower neutralizing capacity of asymmetric molecules was demonstrated in vivo.

CONCLUSION: Results show that during pregnancy there is a modulation of the immune response with an increase in the production of asymmetric molecules of lower protective capacity.

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