T-Helper 2 Type Cytokine and Soluble Interleukin-2 Receptor Levels in Seminal Plasma of Infertile Men

Authors

  • Dr. Roberto Paradisi,

    Corresponding author
    1. Gynaecological Endocrinology Unit, Department of Obstetrics, Gynaecology and Reproductive Biology, S. Orsola Hospital, University Alma Mater Studiorum of Bologna, Bologna, Italy
      Gynaecological Endocrinology Unit, Department of Obstetrics, Gynaecology and Reproductive Biology, S. Orsola Hospital, University Alma Mater Studiorum of Bologna, Massarenti 13, 1-40138 Bologna, Italy.
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  • Rita Mancini,

    1. Central Laboratory, Malpighi Hospital, Bologna, Italy
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  • Emanuela Bellavia,

    1. Gynaecological Endocrinology Unit, Department of Obstetrics, Gynaecology and Reproductive Biology, S. Orsola Hospital, University Alma Mater Studiorum of Bologna, Bologna, Italy
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  • Elisabetta Beltrandi,

    1. Central Laboratory, Malpighi Hospital, Bologna, Italy
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  • Annalisa Pession,

    1. Department of Experimental Pathology; University Alma Mater Studiorum of Bologna, Bologna, Italy
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  • Stefano Venturoli,

    1. Gynaecological Endocrinology Unit, Department of Obstetrics, Gynaecology and Reproductive Biology, S. Orsola Hospital, University Alma Mater Studiorum of Bologna, Bologna, Italy
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  • Carlo Flamigni

    1. Gynaecological Endocrinology Unit, Department of Obstetrics, Gynaecology and Reproductive Biology, S. Orsola Hospital, University Alma Mater Studiorum of Bologna, Bologna, Italy
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Gynaecological Endocrinology Unit, Department of Obstetrics, Gynaecology and Reproductive Biology, S. Orsola Hospital, University Alma Mater Studiorum of Bologna, Massarenti 13, 1-40138 Bologna, Italy.

Abstract

PROBLEM: The role of cell-mediated immunity (CMI) in unexplained male infertility and impaired sperm function has been explored.

METHOD OF STUDY: The presence of cytokines, namely, interleukin-4 (IL-4), interleukin-6 (IL-6), and the soluble interleukin-2 receptor (SIL-2R), was investigated in seminal plasma of 18 fertile and 20 infertile subjects, using specific enzyme-linked immunoadsorbent assays. RESULTS: IL-4 was not detected. SIL-2R was detected, but the concentration difference between the fertile and infertile group was not significant. IL-6 was detected with significantly higher levels in the infertile group compared to the fertile group. IL-6 levels in seminal plasma correlated positively with leukocyte count and negatively with sperm count, motility, and morphology.

CONCLUSIONS: These findings show: a) a lack of IL-4 in seminal plasma; b) similar SIL-2R levels in fertile and infertile seminal plasma; c) increased IL-6 secretion in seminal plasma of infertile subjects; and d) specific correlations of IL-6 with the main semen parameters.

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