The Anovulation in Female Mice Resulting from Postnatal Injections of Estrogen is Correlated with Altered Levels of CD8+ Lymphocytes

Authors

  • Rohini R. Deshpande,

    1. Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York
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  • John C. Chapman,

    1. Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York
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  • Sandra D. Michael

    Corresponding author
    1. Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York
      Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13902-6000.
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Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13902-6000.

Abstract

PROBLEM: Injections of estradiol-17β (E2) are known to both induce anovulation and alter lymphocyte maturation in female mice. The current study examined whether the two events are related.

METHOD OF STUDY: Female (C3H/HeJ × 129J)F1 (C31) mice were injected with 20 μg of E2 from 0–3 days, or from 3–6 days, postpartum. At 8, 12, 20, 32, or 40 weeks of age, the animals were killed, T lymphocytes were characterized, and ovaries were histologically examined for the presence of corpora lutea.

RESULTS: Animals injected with E2 from 0–3 days postpartum had percentages of CD8+ thymocytes and CD8+ splenocytes that were always lower than in noninjected females, and the E2-injected animals never ovulated, even by 40 weeks of age. In contrast, animals injected with E2 from 3–6 days of age had percentages of CD8+ thymocytes and CD8+ splenocytes that, although initially lower than in control females, attained control values by 32 weeks of age. In addition, at 32 weeks of age a number of the 3–6-day E2-injected females ovulated, whereas at earlier ages none had. Further, injections of E2 had little effect on the percentages of CD4+ thymocytes and splenocytes in these animals.

CONCLUSIONS: The results suggest that E2-induced anovulation in C31 female mice is correlated with decreased levels of CD8+ lymphocytes, and an increased CD4+/CD8+ lymphocyte ratio.

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