Expression of TJ6 During Pregnancy


Department of Microbiology and Immunology, Finch University of Health Sciences/The Chicago Medical School, 333 Green Bay Road, North Chicago, IL 60064


PROBLEM: TJ6 will be one of the molecules involved in fetal-specific immune suppression during pregnancy. In the mouse and human decidua, the regulation of uterine natural killer (uNK) cells is important during pregnancy.

METHOD OF STUDY: To further understand the possible functions of TJ6 during pregnancy, syngeneic, allogeneic, and mutant mice were examined for TJ6 expression. RESULTS: Immunoblotting showed that TJ6 protein was expressed on most of the placenta-associated mononuclear cells, and the size was 70–72 kDa at all stages of pregnancy. The expression of TJ6 mRNA was studied by a ribonuclease protection assay in syngeneic and allogeneic matings, and in immune-deficient mice of genotypes scid/scid and scid/scid. bg/bg.

CONCLUSIONS: Genetic disparity, lack of T and B lymphocytes, and loss of NK lytic function had no significant effect on the expression of TJ6 mRNA.