Characterization of Antibodies Generated Against a Conserved Portion of Oviductal Glycoprotein (OGP) and Endogenous Hamster OGP and Their Ability to Decrease Sperm Binding to the Zona Pellucida In Vitro
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 38, Issue 6, pages 377–383, December 1997
How to Cite
Schmidt, A., Mavrogianis, P.A., O'Day-Bowman, M.B., Jaffe, M.C. and Verhage, H.G. (1997), Characterization of Antibodies Generated Against a Conserved Portion of Oviductal Glycoprotein (OGP) and Endogenous Hamster OGP and Their Ability to Decrease Sperm Binding to the Zona Pellucida In Vitro. American Journal of Reproductive Immunology, 38: 377–383. doi: 10.1111/j.1600-0897.1997.tb00316.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted May 20, 1997
- oviductal glycoprotein;
- sperm binding;
- zona pellucida
PROBLEM: The effect of antibodies generated against hamster oviductal glycoprotein (OGP) on sperm binding to the zona pellucida (ZP) was evaluated.
METHOD OF STUDY: Antibodies against a 17-amino-acid sequence of the OGP core protein (amino acids 52–68) and the denatured hamster OGP protein were generated, characterized, and tested in an in vitro sperm binding assay.
RESULTS: Sperm binding was significantly decreased (P < 0.05) when oviductal oocytes were incubated for 2 hr with 4 or 8 mg/ml of immune IgG of both antibodies when compared with normal rabbit IgG. A fluorescence assay showed binding of both antibodies to the endogenous OGP associated with the ZP of ovulated hamster oocytes. CONCLUSIONS: These results suggest that OGP may be a potential immunocontraceptive target because both antibodies significantly decreased sperm binding to the ZP of oviductal oocytes. Immunocontraception may be accomplished by attempting to generate active immunity to a recombinant OGP, to the region selected in this study (amino acids 52–68) or to some other region of the core protein.