Transferrin Receptor (CD71) Expression in Peritoneal Macrophages from Fertile and Infertile Women With and Without Endometriosis

Authors

  • Sergio Martínez-Román,

    1. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
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  • Dr. Juan Balasch,

    Corresponding author
    1. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
      Department of Obstetrics and Gynecology Hospital Clinic i Provincial, C/Casanova 143, 08036-Barcelona, Spain.
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  • Montserrat Creus,

    1. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
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  • Francisco Fábregues,

    1. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
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  • Francisco Carmona,

    1. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
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  • Ramón Vilella,

    1. Immunology Service, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
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  • Juan A. Vanrell

    1. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Barcelona, Hospital Clinic i Provincial, Barcelona, Spain
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Department of Obstetrics and Gynecology Hospital Clinic i Provincial, C/Casanova 143, 08036-Barcelona, Spain.

Abstract

PROBLEM: Hyperactivated macrophages are implicated in the pathophysiology of endometriosis-associated infertility. This study investigates transferrin receptor expression (CD71) as a marker of hyperactivity in peritoneal macrophages of infertile patients with minimal to mild endometriosis (group 1, n = 25).

METHOD OF STUDY: Expression of the activation antigen CD71 on peritoneal fluid macrophages was determined by a specific monoclonal anti-CD71 antibody using indirect immunofluorescence technique and was analyzed by flow cytometry. Three different control groups of women were used: women with unexplained infertility (group 2, n = 25), fertile women with endometriosis (group 3, n =10), and fertile women without endometriosis (group 4, n = 25).

RESULTS: The percentage of CD71 positive cells was significantly increased in infertile women with endometriosis as compared with the three control groups. There were no differences among groups 2, 3, and 4 with respect to the percentage of CD71 positive macrophages.

CONCLUSIONS: Our results favor the concept that hyperactivated macrophages play a role in the pathophysiology of endometriosis-associated subfertility, a feature which is lacking in patients with unexplained infertility.

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