Soluble Human Leukocyte Antigens, Interleukin-6, and Interferon-γ During Pregnancy

Authors

  • G.P. Russwurm,

    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
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  • A.M. Mackler,

    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
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  • O.R. Fagoaga,

    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
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  • W.S. Brown III,

    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
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  • E.P. Sakala,

    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
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  • S.M. Yellon,

    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
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  • S.L. Nehlsen-Cannarella

    Corresponding author
    1. Immunology Center, Center for Perinatal Biology, Departments of Pathology, Physiology, and Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, CA
      Immunology Center, Department of Pathology, Loma Linda University School of Medicine, Loma Linda, CA 92350.
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Immunology Center, Department of Pathology, Loma Linda University School of Medicine, Loma Linda, CA 92350.

Abstract

PROBLEM: Soluble human leukocyte antigens (sHLA), interferon-γ (IFN-γ), and interleukin-6 (IL-6) were studied during human pregnancy to test the hypothesis that sHLA concentrations are regulated by these specific cytokines.

METHOD OF STUDY: Enzyme-linked immunoadsorbent assays (ELISA) were used to measure sHLA I and II in maternal circulation, cord blood, and placenta effluents of pregnant and nonpregnant women; maternal serum cytokines were also determined.

RESULTS: sHLA in maternal and cord blood were equivalent to that in the placenta. By the third trimester, sHLA I concentrations in maternal plasma were significantly reduced compared to the first or second trimesters. sHLA II was increased during the second trimester relative to that postpartum. Maternal IL-6 and IFN-γ concentrations were not statistically different throughout gestation or postpartum.

CONCLUSIONS: These data do not suggest a role for maternal plasma IL-6 or IFN-γ in regulation of systemic sHLA class I during pregnancy, but they do not address whether such events take place in local tissues of the maternal-fetal unit.

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