Regulation of Interleukin (IL)-6 and IL-8 Production in an Amnion-derived Cell Line by Cytokines, Growth Factors, Glucocorticoids, and Phorbol Esters
Version of Record online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 38, Issue 4, pages 272–278, October 1997
How to Cite
Keelan, J. A., Sato, T. and Mitchell, M. D. (1997), Regulation of Interleukin (IL)-6 and IL-8 Production in an Amnion-derived Cell Line by Cytokines, Growth Factors, Glucocorticoids, and Phorbol Esters. American Journal of Reproductive Immunology, 38: 272–278. doi: 10.1111/j.1600-0897.1997.tb00514.x
- Issue online: 6 SEP 2011
- Version of Record online: 6 SEP 2011
- Accepted accepted May 5, 1997
PROBLEM: To determine whether amnion cells produce interleukin (IL)−6 and −8 and thus may contribute to the high concentrations of these cytokines in amniotic fluid at term.
METHOD OF STUDY: Amnion-derived WISH cells were treated in culture with stimuli over 16 hr, and IL-6 and IL-8 concentrations in the conditioned media were measured by enzyme-linked immunosorbent assay or bioassay (IL-6 only).
RESULTS: IL-8 production was ˜5-fold higher than that of IL-6 under basal and stimulated conditions. Significant (by Dunnett's test after analysis of variance) stimulation of production of both cytokines was achieved by IL-1β (>0.2 ng/ml), TNFα (>10 ng/ml), and the phorbol ester, phorbol 12-myristate 13-acetate (>2 nM), over a 16-hr culture period. Epidermal growth factor at 10 ng/ml induced a small increase in production of IL-8, but not of IL-6, whereas bacterial lipopolysaccharide had minimal effects on production of either cytokine. Basal and cytokine-stimulated IL-6 and IL-8 production was inhibited by dexamethasone at concentrations equal to or greater than 1 nM.
CONCLUSION: These findings suggest that amnion may be a significant contributor to the IL-6 and IL-8 content of amniotic fluid, and that WISH cells may be a suitable model for the study of cytokine production by amnion epithelial cells.