Cytokine Expression by First-Trimester Human Chorionic Villi
Version of Record online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 40, Issue 5, pages 309–318, November 1998
How to Cite
Bennett, W.A., Lagoo-Deenadayalan, S., Stopple, J.A., Barber, W.H., Hale, E., Brackin, M. N. and Cowan, B. D. (1998), Cytokine Expression by First-Trimester Human Chorionic Villi. American Journal of Reproductive Immunology, 40: 309–318. doi: 10.1111/j.1600-0897.1998.tb00059.x
- Issue online: 6 SEP 2011
- Version of Record online: 6 SEP 2011
- accepted March 18, 1998
- growth factor;
- messenger ribonucleic acid
PROBLEM: Communication at the human maternal-fetal interface occurs by an intricate cytokine network. This study examines cytokine expression by normal first-trimester human chorionic villi.
METHOD OF STUDY: Tissues were obtained at elective pregnancy terminations (7–9 weeks). Total RNA was isolated from chorionic villi by guanidinium isothiocynate-acid phenol extraction. A reverse transcriptase-polymerase chain reaction technique was used to examine cytokine expression. β-Actin was used as the housekeeping gene, and mitogen-stimulated lymphocytes served as positive controls.
RESULTS: β-Actin was uniformly expressed by all chorionic villous samples. Interferon (IFN)-α and -β also were highly expressed. Moderate expression was noted for interleukin (IL)-10, IL-6, tumor necrosis factor (TNF)-α, and IL-1β. In contrast, transforming growth factor-β1, IFN-γ, IL-2, and IL-1α were either weakly expressed or absent in first-trimester villi. CONCLUSIONS: Cytokines may contribute to pregnancy immunotolerance (IFN-α, IFN-β, and IL-10), viral resistance (IFNs), hormone secretion (IL-1 and IL-6), and cellular remodeling (IFN-γ and TNF-α) within the chorionic villous.