Endothelin-1: Expression and Role in Human Corpus Luteum
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 40, Issue 5, pages 370–376, November 1998
How to Cite
Apa, R., Miceli, F., Feo, D. D., Pierro, E., Ayala, G., Mancuso, S., Napolitano, M. and Lanzone, A. (1998), Endothelin-1: Expression and Role in Human Corpus Luteum. American Journal of Reproductive Immunology, 40: 370–376. doi: 10.1111/j.1600-0897.1998.tb00068.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- accepted July 1, 1998
- Corpus luteum;
PROBLEM: Several recent data suggest an involvement of endothelin (ET)-1, a powerful vasoconstrictor peptide, in reproductive function. This study was designed to investigate the presence and role of ET-1 in human corpus luteum.
METHOD OF STUDY: Purified luteal cells were incubated for different times with ET-1 or ET-3 alone or associated with human chorionic gonadotropin. In another set of experiments cells were treated with ET-1 and BQ485, an ET-A receptor antagonist, or with phorbol 12-myristate-13 acetate (PMA), an activator of protein kinase C.
RESULTS: ET-1 reduced both basal and human chorionic gonadotropin-induced progesterone production at all examined times, similarly PMA inhibited basal progesterone synthesis. BQ485 prevented the inhibitory effect of ET-1, while no effect was observed with ET-3. Finally, ET-1 mRNA was detected in the luteal cells.
CONCLUSION: ET-1 is expressed by human luteal cells and reduces basal and human chorionic gonadotropin-induced progesterone synthesis through the ET-A receptors and the protein kinase C pathway. Conversely, ET-3 does not affect luteal steroidogenesis.