Endometrial Leukocytes Are Altered Numerically and Functionally in Women with Implantation Defects
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 39, Issue 1, pages 1–11, January 1998
How to Cite
Stewart-Akers, A. M., Krasnow, J. S., Brekosky, J. and Deloia, J. A. (1998), Endometrial Leukocytes Are Altered Numerically and Functionally in Women with Implantation Defects. American Journal of Reproductive Immunology, 39: 1–11. doi: 10.1111/j.1600-0897.1998.tb00326.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted July 23, 1997
- Endometrial leukocytes;
- T cells;
- Thl cytokines;
- Th2 cytokines
PROBLEM: A significant cohort of women with autoimmune thyroid disease (ATD) also suffer from reduced fertility. The finding that neither exogenous hormones nor donor eggs correct the infertility suggests that the problem involves an inherent endometrial defect.
METHOD OF STUDY: Endometrial leukocyte populations in women with ATD were quantitated by immunohistochemistry. A semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to examine the expression of transforming growth factor (TGF)-β1, interleukin (IL)-4, IL-10, and interferon (IFN)-γ in the endometrial samples.
RESULTS: A significant increase in the endometrial T-cell population was observed in women with ATD compared to controls. The relative abundance of IL-4 and IL-10 was decreased in women with ATD compared to controls, whereas IFN-γ was increased. No difference was noted in the abundance of TGF-β1. The source of cytokine production for IL-4, IL-10, and IFNγ was the endometrial leukocytes.
CONCLUSIONS: Both the leukocyte numbers and cytokine expression profile were altered significantly in a well-defined group of women with implantation defects.