Incidence of Rat-Soluble Placental Factors on IgE and IgG2a Synthesis

Authors

  • Dr. Teresa Gentile,

    Corresponding author
    1. Instituto de Estudios de la Inmunidad Humoral (CONICETUBA), Departamento de Microbiología, Cátedra de Matemática, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
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  • Jose Dokmetjian,

    1. Instituto de Estudios de la Inmunidad Humoral (CONICETUBA), Departamento de Microbiología, Cátedra de Matemática, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
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  • Delia Garrido,

    1. Instituto de Estudios de la Inmunidad Humoral (CONICETUBA), Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Matemática, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
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  • R.A. Margni

    1. Instituto de Estudios de la Inmunidad Humoral (CONICETUBA), Departamento de Microbiología, Cátedra de Matemática, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
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IDEHU, Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, Junín 956, 1113 Buenos Aires, Argentina.

Abstract

PROBLEM: The in vivo effect of soluble factors present in placental culture supernatants (PCSs) on the synthesis of rat immunoglobulin E (IgE) and IgG2a isotypes was investigated.

METHOD OF STUDY: Batches of Wistar SPF rats immunized with a 10-μg dose of ovalbumin and Al(OH)3 were used: group I, consisted of virgin rats; group II, virgin females injected simultaneously with PCSs; and group III, pregnant females. As controls, nonimmunized batches were included. Serum samples were collected at days 0 (basal) and 10 after antigen challenge, determining levels of total and specific antiovalbumin of both IgE and IgG2a by enzyme-linked immunoadsorbent assay (ELISA).

RESULTS: In vivo and at least at the doses administered, PCSs exert an inhibitory effect on the synthesis of specific and total anti-ovalbumin IgE during the course of immune response to such challenge. However, PCSs did not modify serum values of total and specific IgG2a.

CONCLUSIONS: These results suggest that PCSs exert selective influence on the synthesis of diverse immunoglobulin isotypes during immune response, through the balance of cytokines synthesized by placental cells.

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