The Peritoneal Fluid Levels of Interleukin-12 in Women with Endometriosis
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 39, Issue 2, pages 152–156, February 1998
How to Cite
Zeyneloglu, H. B., Senturk, L. M., Seli, E., Bahtiyar, O. M., Olive, D. L. and Arici, A. (1998), The Peritoneal Fluid Levels of Interleukin-12 in Women with Endometriosis. American Journal of Reproductive Immunology, 39: 152–156. doi: 10.1111/j.1600-0897.1998.tb00347.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted July 25, 1997
- peritoneal fluid
PROBLEM: Interleukin-12 (IL-12) is produced mainly by monocytes/macrophages, and it induces proliferation and cytotoxicity of T-cells and natural killer cells. In women with endometriosis, natural killer cell activity in the peritoneal fluid is significantly decreased. We aimed to measure the peritoneal fluid level of IL-12 in endometriosis.
METHOD OF STUDY: We measured IL-12 levels in peritoneal fluid samples from women with or without endometriosis and in supernatants from endometrial stromal, ovarian stromal, and mesothelial cell cultures, using a high-sensitivity enzyme-linked immunosorbent assay.
RESULTS: The median concentration of IL-12 in the peritoneal fluid of women with endometriosis was 1.1 pg/ml (range, 0.2–5.5) and was 1.6 pg/ml (range, 0.4-2.8) in women without endometriosis, not a statistically significant difference. IL-12 was not detected in the supernatants of endometrial stromal, ovarian stromal, and mesothelial cell cultures. CONCLUSION: Concentrations of IL-12 in the peritoneal fluid of women with or without endometriosis are low, but they are detectable and are not affected significantly by the presence of endometriosis.