The Herbal Medicine Unkei-To Stimulates Cytokine-Induced Neutrophil Chemoattractant Production in the Pituitary Folliculo-Stellate—Like Cell Line (TtT/GF)
Version of Record online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 39, Issue 4, pages 249–255, April 1998
How to Cite
Koike, K., Zhang, Z. X., Sakamoto, Y., Jikihara, H., Masuhara, K., Murakami, K., Miyake, A. and Inoue, M. (1998), The Herbal Medicine Unkei-To Stimulates Cytokine-Induced Neutrophil Chemoattractant Production in the Pituitary Folliculo-Stellate—Like Cell Line (TtT/GF). American Journal of Reproductive Immunology, 39: 249–255. doi: 10.1111/j.1600-0897.1998.tb00361.x
- Issue online: 6 SEP 2011
- Version of Record online: 6 SEP 2011
- Accepted September 16, 1997
- Anterior pituitary gland;
- cytokine-induced neutrophil chemoattractant;
- folliculo-stellate cell;
- herbal medicine;
PROBLEM: We previously reported that a cytokine-induced neutrophil chemoattractant (CINC) was produced in the pituitary gland and that it influenced anterior pituitary hormone release. In this study we investigated the effect of Unkei-to, a Japanese herbal medicine, on CINC production in the rat anterior pituitary gland and the pituitary folliculo-stellate-like cell line (TtT/GF).
METHOD OF STUDY: Dispersed normal anterior pituitary cells and the folliculo-stellate-like cell line TtT/GF were used to test the effect of Unkei-to on CINC secretion and CINC mRNA accumulation. Concentrations of CINC in the conditioned media were measured by an enzyme-linked immunosorbent assay, and levels of CINC mRNA were analyzed by Northern blot analysis.
RESULTS: Unkei-to (20 μg/ml) significantly increased the secretion of CINC by normal anterior pituitary cells within 12 hr of incubation. Unkei-to also stimulated CINC secretion from TtT/GF cells in a time- and dose-dependent manner. Unkei-to (20 μg/ml) increased CINC mRNA accumulation in TtT/GF cells within 3 hr of incubation and also caused a 13-fold increase in the secretion of CINC from TtT/GF cells compared with the vehicle group within 24 hr of incubation. Finally, we found that some of the Unkei-to's ingredients, Evodiae fructus and Pinelliae tuber, markedly stimulated CINC secretion from TtT/GF cells.
CONCLUSIONS: Our results will help to elucidate the mechanism behind the clinical effect of Unkei-to on the anterior pituitary gland. They also suggested the presence of special substances, which stimulate CINC secretion, within Unkei-to's ingredients such as E. fructus and P. tuber.