Presence of Leukemia Inhibitory Factor and Interleukin-12 in Human Follicular Fluid During Follicular Growth
Version of Record online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 40, Issue 1, pages 13–18, July 1998
How to Cite
Coskun, S., Uzumcu, M., Jaroudi, K., Hollanders, J. M.G., Parhar, R. S. and Al-Sedairy, S. T. (1998), Presence of Leukemia Inhibitory Factor and Interleukin-12 in Human Follicular Fluid During Follicular Growth. American Journal of Reproductive Immunology, 40: 13–18. doi: 10.1111/j.1600-0897.1998.tb00382.x
- Issue online: 6 SEP 2011
- Version of Record online: 6 SEP 2011
- Accepted January 22, 1998
- Follicular fluid;
- human chorionic gonadotropin;
PROBLEM: Cytokines have been shown to be present in human follicular fluid and have regulatory functions on follicular maturation. The presence of leukemia inhibitory factor (LIF) and interleukin (IL)-12 in human follicular fluid obtained at different stages of maturation was investigated.
METHOD OF STUDY: Follicular fluids and granulosa cells were obtained from preovulatory and immature follicles. Follicular fluids from both groups were assayed for IL-12 and LIF by enzyme-linked immunosorbent assay. Granulosa cells from preovulatory and immature follicles were treated with human chorionic gonadotropin (hCG) in vitro and subsequent LIF and IL-12 production were measured.
RESULTS: The average concentration of LIF was significantly higher in preovulatory follicles (7.6 ± 1.3 pg/ml, n = 24) than in immature follicles (2.0 ± 1.3 pg/ml, n = 6). The concentration of IL-12 was significantly higher in follicular fluid obtained from immature follicles (10.9 ± 5.0 pg/ml) than in preovulatory follicles (1.3 ± 0.4 pg/ml). hCG only stimulated LIF production from mature granulosa cells; it had no effect on IL-12 production.
CONCLUSIONS: IL-12 and LIF are present in follicular fluid and their levels are regulated differently during follicular maturation. hCG stimulates LIF production from granulosa cells in vitro.