Fertility-Disrupting Potential of Synthetic Peptides Derived from the β-Subunit of Follicle-Stimulating Hormone

Authors

  • VALERIE A. FERRO,

    1. University of Strathclyde, Department of Immunology, Glasgow, Scotland
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  • WILLIAM H. STIMSON

    Corresponding author
    1. University of Strathclyde, Department of Immunology, Glasgow, Scotland
      University of Strathclyde, Department of Immunology, The Todd Centre, 31 Taylor Street, Glasgow, G4 ONR Scotland.
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University of Strathclyde, Department of Immunology, The Todd Centre, 31 Taylor Street, Glasgow, G4 ONR Scotland.

Abstract

PROBLEM: Hormone immunoneutralization is hampered by immunologic cross-reactivity caused by close-sequence homology between related molecules. One solution is to use smaller fragments to induce antibodies of greater specificity.

METHOD OF STUDY: A number of peptides selected from β-follicle-stimulating hormone (FSH) were conjugated to tetanus toxoid and were used to immunize female rats. The antis-era were examined for FSH cross-reactivity by immunoassays and in an in vitro bioassay.

RESULTS: In the immunoassays, the antisera did not react with FSH but did react with their respective peptides. In the bioassay, sera from VYKDPARPC- and CDSLYTYP-immunized animals inhibited FSH-receptor interaction by 73% and 68%, respectively. These animals also showed reduced estradiol levels. Sequences were synthesized around VYKDPARPC and were tested on a FSH-receptor-bearing Chinese hamster ovary cell line. LVYKDPARPC, VYKDPARPC, YKDPARPIC, CLVYKDPARP, and LVYKDPARP inhibited FSH-receptor interaction by greater than 50%. In female mice, TRDLVYKDPARPKI and LVYKDPARP disrupted estrous cycling in all animals; LVYKDPARPC and CLVYKDPARP disrupted cycling in three of five animals, whereas VYKDPARPC disrupted cycling in one of four animals.

CONCLUSIONS: Peptides from two areas of β-FSH (VYKDPARP and DSLYTYP) were shown to raise FSH-neutralizing antibodies, which were able to suppress estradiol levels. An additional leucine residue to VYKDPARP greatly enhanced the peptide's ability to inhibit FSH-receptor binding and caused fertility disruption in vivo.

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