Expression and Localization of the Th2-type Cytokine Interleukin-13 and Its Receptor in the Placenta During Human Pregnancy
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 40, Issue 4, pages 283–290, October 1998
How to Cite
DEALTRY, G. B., CLARK, D. E., SHARKEY, A., CHARNOCK-JONES, D. S. and SMITH, S. K. (1998), Expression and Localization of the Th2-type Cytokine Interleukin-13 and Its Receptor in the Placenta During Human Pregnancy. American Journal of Reproductive Immunology, 40: 283–290. doi: 10.1111/j.1600-0897.1998.tb00419.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted February 24, 1998
- cytokine receptors;
PROBLEM: To investigate the expression of the Th2-type cytokine interleukin (IL)-13 and its receptor in human placenta during gestation.
METHOD OF STUDY: Expression of IL-13 and its receptor was analyzed by reverse transcriptase (RT)-polymerase chain reaction (PCR), in situ hybridization and immunohistochemistry using human placental samples.
RESULTS: IL-13 mRNA was detected by RT-PCR in placental extracts from all stages of gestation. In situ hybridization revealed IL-13 mRNA in first trimester cytotrophoblast and syncytiotrophoblast. Few positive cells were found within decidual sections from the same pregnancy. Immunohistochemistry revealed a similar pattern to in situ hybridization. This was largely absent in second- and third-trimester placentae. IL-13 receptor α chain (IL-13Rα) was detected by immunofluorescence on the surface of leukocytes in first-trimester villous core and decidua.
CONCLUSIONS: The expression of IL-13 is spatially and temporally regulated in the placenta, and associated with the close proximity of a receptor-bearing target leukocyte population.