Elevated IL-6 in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 39, Issue 5, pages 329–334, May 1998
How to Cite
Nakabayashi, M., Sakura, M., Takeda, Y. and Sato, K. (1998), Elevated IL-6 in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia. American Journal of Reproductive Immunology, 39: 329–334. doi: 10.1111/j.1600-0897.1998.tb00526.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted August 25, 1997
- Coagulation and fibrinolysis;
- cultured trophoblast cells;
- placental development;
PROBLEM: The primary defect of placental development in preeclampsia is speculated to occur at midtrimester gestation. Abnormal feto-maternal immune reactions have been considered as factors in such defective placentation.
METHOD OF STUDY: Midtrimester amniotic fluid specimens were retrospectively identified as coming from gestations that later had severe preeclampsia develop, gestations with normal outcomes, and gestations measured for cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, IL-6, and IL-8). The effect of each cytokine on thrombomodulin levels was tested in cultured trophoblast cells.
RESULTS: Among the measured cytokines, IL-6 and IL-8 were significantly elevated in the midtrimester amniotic fluid of the future preeclamptic group. Trophoblasts stimulated with TNF-α plus IL-6 had significantly decreased levels of cellular thrombomodulin compared to those without cytokine addition.
CONCLUSIONS: Elevated cytokines in midtrimester amniotic fluid suggest an abnormal fetomaternal immune response occurring before the clinical manifestation of preeclampsia. Cytokine-induced suppression of thrombomodulin in trophoblasts may be directly involved in the pathogenesis of preeclampsia.