Interleukin-6 Levels in Co-culture of Human In Vitro Fertilization Embryos with Vero Cells Are Not Predictive of Future Successful Development

Authors

  • Geneviève Laprée-Delage,

    Corresponding author
    1. INSERM U131, Equipe Cytokines dans la relation materno-foetale, Bâtiment de Gynécologie Obstétrique, Hôpital Antoine Béclère, 157, rue de la Porte de Trivaux. 92141 Clamart Cedex, France
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  • Monique Volante,

    1. Service de Gynécologie/Obstétrique, Hôpital Antoine Béclère, Clamart Cedex, France
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  • René Frydman,

    1. Service de Gynécologie/Obstétrique, Hôpital Antoine Béclère, Clamart Cedex, France
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  • Gérard Chaouat

    1. INSERM U131, Equipe Cytokines dans la relation materno-foetale, Bâtiment de Gynécologie Obstétrique, Hôpital Antoine Béclère, 157, rue de la Porte de Trivaux. 92141 Clamart Cedex, France
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INSERM U131, Equipe Cytokines dans la relation materno-foetale, Bâtiment de Gynécologie Obstétrique, Hôpital Antoine Béclère, 157, rue de la Porte de Trivaux. 92141 Clamart Cedex, France.

Abstract

PROBLEM: In an attempt to predict successful embryo transfer and implantation, we measured interleukin (IL)-6 levels in culture supernatants of co-cultured preimplantation human embryos. We tested whether all in vitro—fertilized human embryos in co-cultures do secrete IL-6, and whether there was any difference in such production between embryos that successfully reached the blastocyst stage and blocked embryos. We also addressed the question of IL-6 secretion by co-culture support cells, namely Vero cells themselves.

METHOD OF STUDY: Each fertilized oocyte was cultured individually and transferred in culture wells supplemented with a feeder layer of Vero cells at day 2. In vitro IL-6 production was measured by bioassay of the culture media.

RESULTS: Because Vero cells themselves secrete IL-6, it became impossible, in co-culture, to quantify production of IL-6 by the sole embryos. On the other hand, the co-culture technique has shown us that embryos are likely to consume IL-6. There was no difference between blastocysts and blocked embryos.

CONCLUSIONS: IL-6 levels in human embryo co-cultures do not correlate with future successful embryo transfer.

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