Possible Susceptibility of the HLA-DPB1*0402 and HLA-DPB1*04 Alleles to Unexplained Recurrent Abortion: Analysis by means of Polymerase Chain Reaction–Restricted Fragment Length Polymorphism Method

Authors

  • Koichi Takakuwa M. D.,

    Corresponding author
    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Isao Hataya,

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Masato Arakawa,

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Akira Kikuchi,

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Masahiko Higashino,

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Masako Yasuda,

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Takumi Kurabayashi,

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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  • Kenichi Tanaka

    1. Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan
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Department of Obstetrics and Gynecology, 1–757, Asahimachi-dori, Niigata, 951–8510, Japan.

Abstract

Takakuwa K, Hataya I, Arakawa M, Kikuchi A, Higashino M, Yasuda M, Kurabayashi T, Tanaka K. Possible susceptibility of the HLA-DPB1*0402 and HLA-DPB1*04 alleles to unexplained recurrent abortion – analysis by means of polymerase chain reaction–restricted fragment length polymorphism method. AJRI 1999; 42:233–239 © Munksgaard, Copenhagen

PROBLEM: To clarify whether HLA-DP antigens are associated with patient population of unexplained recurrent abortion.

METHOD OF STUDY: The frequency of HLA-DPB1 alleles in patients with unexplained recurrent abortion, and the compatibility of HLA-DPB1 alleles between patient couples, were studied using a polymerase chain reaction (PCR)–restricted fragment length polymorphism (RFLP) method. Thirty patients who had a history of unexplained primary recurrent abortion, and their husbands, were typed for HLA-DPB1 genotype. Two hundred and ninety-nine base pair fragments from the second exon of HLA-DPB1 genes were selectively amplified using the PCR-primers. After amplification, the DNAs were digested with restriction endonucleases, and subjected to electrophoresis in a 12% polyacrilamide gel to determine HLA-DPB1 genotype.

RESULTS: The frequency of HLA-DPB1*0402 and DPB1*04 alleles in the patient group (n = 30) was significantly increased, as compared to that in the normal fertile women (n = 30). The frequency of HLA-DPB1*04 allele in the patient group was significantly increased, as compared to that in the general population (n = 112). No significant compatibility of HLA-DPB1 alleles could be observed between patient couples and normal fertile couples.

CONCLUSION: These findings suggest a possible new class II association with patient population of unexplained recurrent abortion.

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