Takakuwa K, Hataya I, Arakawa M, Kikuchi A, Higashino M, Yasuda M, Kurabayashi T, Tanaka K. Possible susceptibility of the HLA-DPB1*0402 and HLA-DPB1*04 alleles to unexplained recurrent abortion – analysis by means of polymerase chain reaction–restricted fragment length polymorphism method. AJRI 1999; 42:233–239 © Munksgaard, Copenhagen
PROBLEM: To clarify whether HLA-DP antigens are associated with patient population of unexplained recurrent abortion.
METHOD OF STUDY: The frequency of HLA-DPB1 alleles in patients with unexplained recurrent abortion, and the compatibility of HLA-DPB1 alleles between patient couples, were studied using a polymerase chain reaction (PCR)–restricted fragment length polymorphism (RFLP) method. Thirty patients who had a history of unexplained primary recurrent abortion, and their husbands, were typed for HLA-DPB1 genotype. Two hundred and ninety-nine base pair fragments from the second exon of HLA-DPB1 genes were selectively amplified using the PCR-primers. After amplification, the DNAs were digested with restriction endonucleases, and subjected to electrophoresis in a 12% polyacrilamide gel to determine HLA-DPB1 genotype.
RESULTS: The frequency of HLA-DPB1*0402 and DPB1*04 alleles in the patient group (n = 30) was significantly increased, as compared to that in the normal fertile women (n = 30). The frequency of HLA-DPB1*04 allele in the patient group was significantly increased, as compared to that in the general population (n = 112). No significant compatibility of HLA-DPB1 alleles could be observed between patient couples and normal fertile couples.
CONCLUSION: These findings suggest a possible new class II association with patient population of unexplained recurrent abortion.