Presented in part at the 41st Annual Meeting of Society for Gynecologic Investigation, March 1994, Chicago IL.
The Expression of Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) and Receptors in Human Endometrium*
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 42, Issue 5, pages 303–311, November 1999
How to Cite
Zhao, Y. and Chegini, N. (1999), The Expression of Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) and Receptors in Human Endometrium. American Journal of Reproductive Immunology, 42: 303–311. doi: 10.1111/j.1600-0897.1999.tb00106.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- accepted April 20, 1999.
Zhao Y, Chegini N. The expression of granulocyte macrophage-colony stimulating factor (GM-CSF) and receptors in human endometrium. AJRI 1999; 42:303–311 © Munksgaard, Copenhagen
PROBLEM: To determine the temporal and spatial expression of granulocyte macrophage-colony stimulating factor (GM-CSF) and GM-CSF α and β receptor mRNA and protein in human endometrium.
METHOD OF STUDY: The endometrial expression of GM-CSF and GM-CSF receptor mRNA and protein was determined using competitive quantitative reverse transcription polymerase chain reaction (Q-RT-PCR), in situ hybridization, and immunohistochemistry.
RESULTS: Endometrium expresses GM-CSF and GM-CSF α receptor mRNA with maximal expression occurring during the mid-secretory phase (21.1 ±4.2 and 32.2 ± 7.7 × 106 mRNA copies/μg total RNA) compared to the proliferative phase (1.46 ± 0.4 and 7.5 ± 0.5 × 106 copies) of the menstrual cycle, with a significant reduction (0.67 ± 0.1 and 1.7 ± 0.2 × 106 mRNA copies) during the post-menopausal period (P < 0.05). The endometrium expresses a significantly lower level of GM-CSF β receptor mRNA (≈ 0.01 × 105 mRNA copies). Endometrial luminal and glandular epithelial cells are the primary site of GM-CSF mRNA and protein expression, while arteriole endothelial, stromal, and inflammatory cells are the primary site of GM-CSF α receptor protein. GM-CSF β receptor protein has a similar cellular distribution as GM-CSF.
CONCLUSION: Temporal and spatial expression of GM-CSF and GM-CSF receptors in human endometrium during the menstrual cycle suggests that epithelial-derived GM-CSF in an autocrine/paracrine manner may influence various endometrial biological activities, local inflammatory response, and macrophage survival.