Interleukin-8 Stimulates Placental Prostacyclin Production in Preeclampsia


Louisiana State University Medical Center, Department of Obstetrics and Gynecology, PO Box 33932, Shreveport, LA 71130.


Wang Y, Baier J, Adair CD, Lewis DF, Krueger S, Kruger T, Gurski M, Brown E. Interleukin-8 stimulates placental prostacyclin production in preeclampsia. AJRI 1999; 42:375–380 © Munksgaard, Copenhagen

PROBLEM: Our purpose was to determine placental interleukin (IL)-8 production and its correlation with the prostacyclin production in normal and preeclamptic pregnancies and to evaluate the beneficial effect of IL-8 on prostacyclin production.

METHOD OF STUDY: We determined 1) the in vitro production of IL-8 and prostacyclin by placental villous tissues from normal and preeclamptic pregnancies and 2) the production of prostacyclin by villous tissues from preeclampsia treated with recombinant human IL-8 (rhIL-8). IL-8 levels were measured by enzyme-linked immunosorbent assay and prostacyclin by radioimmunoassay of 6-keto PGF1α, the stable metabolite of prostacyclin.

RESULTS: 1) Placental production of IL-8 and 6-keto PGF1α were significantly less in preeclampsia than in normal pregnancies, P < 0.05. 2) Placental production of 6-keto PGF1α and IL-8 was significantly correlated in preeclampsia, P < 0.01. 3) Placental tissues treated with IL-8 exhibited a concentration-dependent increase in 6-keto PGF1α production.

CONCLUSIONS: Placental tissues from preeclampsia produce significantly less IL-8 than tissues from normal pregnancies, which correlates with decreased prostacyclin production. IL-8 improves placental prostacyclin production in preeclampsia.