Wang Y, Baier J, Adair CD, Lewis DF, Krueger S, Kruger T, Gurski M, Brown E. Interleukin-8 stimulates placental prostacyclin production in preeclampsia. AJRI 1999; 42:375–380 © Munksgaard, Copenhagen
PROBLEM: Our purpose was to determine placental interleukin (IL)-8 production and its correlation with the prostacyclin production in normal and preeclamptic pregnancies and to evaluate the beneficial effect of IL-8 on prostacyclin production.
METHOD OF STUDY: We determined 1) the in vitro production of IL-8 and prostacyclin by placental villous tissues from normal and preeclamptic pregnancies and 2) the production of prostacyclin by villous tissues from preeclampsia treated with recombinant human IL-8 (rhIL-8). IL-8 levels were measured by enzyme-linked immunosorbent assay and prostacyclin by radioimmunoassay of 6-keto PGF1α, the stable metabolite of prostacyclin.
RESULTS: 1) Placental production of IL-8 and 6-keto PGF1α were significantly less in preeclampsia than in normal pregnancies, P < 0.05. 2) Placental production of 6-keto PGF1α and IL-8 was significantly correlated in preeclampsia, P < 0.01. 3) Placental tissues treated with IL-8 exhibited a concentration-dependent increase in 6-keto PGF1α production.
CONCLUSIONS: Placental tissues from preeclampsia produce significantly less IL-8 than tissues from normal pregnancies, which correlates with decreased prostacyclin production. IL-8 improves placental prostacyclin production in preeclampsia.