Human Decidualized Endometrial T Lymphocytes Do Not Substantially Down-Regulate CD3ζ
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 41, Issue 4, pages 245–252, April 1999
How to Cite
Bedenicki, I., Newton, D. J., Flanagan, B. F., Johnson, P. M., Rukavina, D. and Christmas, Dr. S. E. (1999), Human Decidualized Endometrial T Lymphocytes Do Not Substantially Down-Regulate CD3ζ. American Journal of Reproductive Immunology, 41: 245–252. doi: 10.1111/j.1600-0897.1999.tb00434.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted September 10, 1998
- reproductive immunology;
- T-cell receptors;
- T lymphocytes
PROBLEM: The T-cell antigen receptor (TCR) has been reported to be down-regulated on T-cells in the decidualized endometrium in early pregnancy.
METHOD OF STUDY: The expression of CD3ζ, a component of the TCR complex, has been investigated in human first-trimester decidual T-cells using flow cytometric analysis of permeabilized cells.
RESULTS: Levels of CD3ζ expression were significantly lower in decidual than in peripheral T-cells from non-pregnant women, as assessed by mean fluorescence intensity (4.2 vs. 5.5. logarithmic scale, P < 0.05). However, when decidual and peripheral T-cells from the same subjects were analyzed (n = 10), mean levels of CD3ζ were slightly, but not significantly, lower in decidual than in peripheral T-cells (P > 0.1). CD3ζ was not substantially down-regulated systemically as mean cytoplasmic CD3ζ levels did not differ significantly between peripheral blood T-cells from pregnant women and non-pregnant controls (P > 0.2). CD8+ cells outnumber CD4+ cells in decidua, but neither the proportions of these two T-cell subsets positive for cytoplasmic CD3ζ nor the mean levels of CD3ζ were significantly different.
CONCLUSIONS: These results indicate that human decidual T-cells do not greatly down-regulate CD3ζ, but it is unclear if a small decrease in mean levels may be sufficient to compromise their capacity for activation.