PROBLEM: To examine factors affecting mouse granulated metrial gland cell cytotoxicity.
METHOD OF STUDY: Chromium-release assays using mouse metrial gland cell suspensions targeted against Wehi 164 fibrosarcoma cells were used. Modulation of cellular cytotoxicity was investigated using an antibody. anti-asialo-GM1, to attempt depletion of effector cells and sera to determine if inhibitory factors are present in blood in vivo.
RESULTS: No reduction in cellular cytotoxicity was found following treatment of effector cells with asialo-GM1 antibody, but there was a reduction following treatment with asialo-GM1 antibody plus guinea pig serum and with guinea pig serum alone. Sera from pregnant, male, and SCID mice also reduced the level of cytotoxicity by metrial gland cell suspensions.
CONCLUSIONS: The reduction in cytotoxicity in the presence of serum indicates that there are factors in the blood which are inhibitory to granulated metrial gland cell cytotoxic activity in vivo. The resistance of mouse metrial gland cells to asialo-GM1 antibody depletion is supportive of a natural cytotoxic (NC) lineage for this cell type.