Interleukin-12 Augments Cytolytic Activity of Peripheral and Decidual Lymphocytes Against Choriocarcinoma Cell Lines and Primary Culture Human Placental Trophoblasts
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 41, Issue 5, pages 320–329, May 1999
How to Cite
Hayakawa, S., Nagai, N., Kanaeda, T., Karasaki-Suzuki, M., Ishii, M., Chishima, F. and Satoh, K. (1999), Interleukin-12 Augments Cytolytic Activity of Peripheral and Decidual Lymphocytes Against Choriocarcinoma Cell Lines and Primary Culture Human Placental Trophoblasts. American Journal of Reproductive Immunology, 41: 320–329. doi: 10.1111/j.1600-0897.1999.tb00445.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- Accepted October 12, 1998
PROBLEM: Human trophoblasts are tolerant to the maternal immune system, but susceptible to interleukin (IL)-2-activated lymphocytes. IL-12 is also a key cytokine in the induction of cytotoxic responses. We administered IL-12 to peripheral blood lymphocytes (PBLs) and to decidual lymphocytes (DLs) and studied resulting cytotoxicity against trophoblasts.
METHOD OF STUDY: PBLs and DLs were stimulated with rIL-2 and/or rIL-12 for 48 hr in vitro. Cytotoxicity against the choriocarcinoma cell line JEG-3, JAR, and primary culture trophoblasts were examined by LDH release assay. The proliferative response was estimated by MTT assay. Expression of cytotoxic factors was studied by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS: Whereas IL-12 alone produced a modest enhancement in cytotoxicity of PBLs and DLs, the combination of IL-2 and IL-12 was most effective in trophoblast cell lysis. IL-12 enhanced the mRNA expression of T-cell specific serine protease (TSP, granzyme B) and FasL in DLs, but the expression of perforin was unchanged. Expression of these cytotoxic factors in PBLs was up-regulated by IL-12.
CONCLUSION: Our findings indicate critical roles of IL-12 in the activation of maternal lymphocytes, which could possibly result in pregnancy failure syndromes.