Mouse Granulated Metrial Gland Cell Cytotoxicity of Wehi 164 Cells: Is There a Role for Interleukin-3 and Tumour Necrosis Factor-α?
Article first published online: 6 SEP 2011
American Journal of Reproductive Immunology
Volume 41, Issue 6, pages 423–427, June 1999
How to Cite
Stewart, I.J. and Peel, S. (1999), Mouse Granulated Metrial Gland Cell Cytotoxicity of Wehi 164 Cells: Is There a Role for Interleukin-3 and Tumour Necrosis Factor-α?. American Journal of Reproductive Immunology, 41: 423–427. doi: 10.1111/j.1600-0897.1999.tb00457.x
- Issue published online: 6 SEP 2011
- Article first published online: 6 SEP 2011
- December 16, 1998
- granulated metrial gland cell;
- NC cell;
PROBLEM: Mouse granulated metrial gland (GMG) cells are maternal, cytotoxic cells found in the uterine wall in pregnancy. In vivo, they are cytotoxic to layer 1 labyrinthine placental cells. In an in vitro system, granulated metrial gland cells are highly cytotoxic to Wehi 164 fibrosarcoma cells. In this study the role of interleukin (IL)-3 and tumour necrosis factor (TNF)-α in granulated metrial gland cell cytotoxic activity was investigated.
METHOD OF STUDY: Chromium-release cytotoxicity assays using mouse metrial gland effector cells and Wehi 164 target cells with the addition of IL-3 or an antibody to TNF-α.
RESULTS: An antibody to TNF-α reduced the level of cytotoxic activity. IL-3 had no effect on the level of cytotoxicity.
CONCLUSION: Mouse granulated metrial gland cells kill using a TNF-α mediated mechanism which is independent of IL-3 stimulation.