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Keywords:

  • Cytotoxicity;
  • granulated metrial gland cell;
  • IL-3;
  • mouse;
  • NC cell;
  • TNF-α

PROBLEM: Mouse granulated metrial gland (GMG) cells are maternal, cytotoxic cells found in the uterine wall in pregnancy. In vivo, they are cytotoxic to layer 1 labyrinthine placental cells. In an in vitro system, granulated metrial gland cells are highly cytotoxic to Wehi 164 fibrosarcoma cells. In this study the role of interleukin (IL)-3 and tumour necrosis factor (TNF)-α in granulated metrial gland cell cytotoxic activity was investigated.

METHOD OF STUDY: Chromium-release cytotoxicity assays using mouse metrial gland effector cells and Wehi 164 target cells with the addition of IL-3 or an antibody to TNF-α.

RESULTS: An antibody to TNF-α reduced the level of cytotoxic activity. IL-3 had no effect on the level of cytotoxicity.

CONCLUSION: Mouse granulated metrial gland cells kill using a TNF-α mediated mechanism which is independent of IL-3 stimulation.