Pregnancy-Specific Glycoprotein Gene Expression in Recurrent Aborters: A Potential Correlation to Interleukin-10 Expression

Authors

  • Lori L. Arnold,

    1. Department of Obstetrics and Gynecology, Georgetown University Medical Center. Washington DC. 20007
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  • T. Mark Doherty,

    1. National Institute of Allergy and Infectious Disease. National Institutes of Health. Bethesda, MD, 20892
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  • Armando W. Flor,

    1. Department of Pediatrics, Georgetown University Medical Center. Washington DC, 20007
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  • James A. Simon,

    1. Department of Obstetrics and Gynecology, Georgetown University Medical Center. Washington DC. 20007
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  • Janice Y. Chou,

    1. National Institute of Child Health and Human Development. National Institutes of Health. Bethesda. MD. 20892
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  • Dr. Wai-Yee Chan,

    Corresponding author
    1. Department of Pediatrics, Georgetown University Medical Center. Washington DC, 20007
    2. Department of Biochemistry and Molecular Biology, and Cell Biology. Georgetown University Medical Center, Washington DC. 20007
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  • Brian C. Mansfield

    1. Department of Pediatrics, Georgetown University Medical Center. Washington DC, 20007
    2. National Institute of Child Health and Human Development. National Institutes of Health. Bethesda. MD. 20892
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    • Human Genome Sciences Inc., 9410 Key West Avenue. Rockville. MD 20850


Department of Pediatrics, Georgetown University Children's Medical Center. 3800 Reservoir Road, NW. Washington DC. 20007

Abstract

PROBLEM: The expression of the pregnancy-specific glycoprotein (PSG) genes in the uterine endometrium of women experiencing recurrent first-trimester abortions, and potential correlations to cytokine expression were examined.

METHOD OF STUDY: Endometrial RNA, isolated from women with a history of either repetitive first-trimester pregnancy losses or uncomplicated pregnancies, was isolated and analyzed for PSG transcripts by the reverse transcriptase-polymerase chain reaction method. PSG genes showing different patterns of expression were expressed in baculovirus, and the purified proteins examined for their effects on cytokine expression.

RESULTS: The expression of PSG11 in the endometria of recurrent aborters was significantly lower than in that of controls (P < 0.01). When tested on monocytes, PSG11 stimulated secretion of interleukin (IL)-10.

CONCLUSIONS: The level of expression of the PSG11 gene in the uterine endometrium, during the peri-implantation period, correlates with the risk of pregnancy loss in some women experiencing recurrent spontaneous abortions. The ability of PSG 11 to influence the secretion of IL-10 suggests that PSG11 may contribute to the local modulation of the inflammatory T helper-1 response seen in the endometrium of these women.

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