Systemic Th1/Th2 Cytokine Responses to Paternal and Vaccination Antigens in Preeclampsia: No Differences Compared with Normal Pregnancy
Article first published online: 16 MAR 2004
American Journal of Reproductive Immunology
Volume 51, Issue 4, pages 302–310, April 2004
How to Cite
Jonsson, Y., Ekerfelt, C., Berg, G., Nieminen, K., Sharma, S., Ernerudh, J. and Matthiesen, L. (2004), Systemic Th1/Th2 Cytokine Responses to Paternal and Vaccination Antigens in Preeclampsia: No Differences Compared with Normal Pregnancy. American Journal of Reproductive Immunology, 51: 302–310. doi: 10.1111/j.1600-0897.2004.00161.x
- Issue published online: 16 MAR 2004
- Article first published online: 16 MAR 2004
- Submitted November 15, 2002; accepted August 12, 2003
Problem: A Th1-shift has been suggested to be involved in the pathogenesis of preeclampsia. This study was designed to compare Th1/Th2 related cytokine secretion in blood between women with preeclampsia (n = 15) and normal pregnancies (n = 15), using a high-sensitivity technique for cytokine detection.
Methods of study: Spontaneous as well as ‘fetus-specific’ and recall antigen-specific (purified protein derivate of Mycobacterium tuberculosis, tetanus toxoid and lipopolysaccharide) secretion of interferon-γ, interleukin (IL)-4, IL-10 and IL-12 in peripheral blood mononuclear cells (PBMC) was detected by enzyme-linked immunosorbent spot-forming cell assay (ELISPOT). Fetus-specific secretion was induced by stimulation with paternal PBMC in a mixed leukocyte culture assay.
Results: All cytokines were secreted by PBMCs both from women with preeclampsia and women with normal pregnancies. No differences in the number of cytokine-secreting cells were found between the two groups.
Conclusions: No evidence was found for a shift in the systemic Th1/Th2 responses, in preeclampsia compared with normal pregnancy. This does, however, not exclude differences in the local immune responses related to the fetoplacental unit.