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Perlecan and Syndecan-4 in Uterine Tissues during the Early Pregnancy in Mice

Authors

  • S. San Martin,

    1. Laboratório de Biologia da Reproducao e da Matriz Extracelular, Departamento de Histologia e Embriologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brasil
    2. Centro de Investigaciones Médicas, Escuela de Medicina, Universidad de Valparaíso, Valparaíso, Chile
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  • M. Soto-Suazo,

    1. Escuela de Medicina, Facultad de Ciencias Médicas, Universidad de Santiago de Chile, Santiago de Chile, Chile
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  • T. M. T. Zorn

    1. Laboratório de Biologia da Reproducao e da Matriz Extracelular, Departamento de Histologia e Embriologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brasil
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Address reprint requests to Prof. Telma M. T. Zorn, Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, CEP: 05508-900, São Paulo, Brazil.
E-mail: temtzorn@usp.br

Abstract

Problem:  During early pregnancy in mice, there is recruitment of specific immune cells, remodeling of the endometrium, cell differentiation and synthesis of new molecules.

Method of study:  Immunohistochemistry was used to determine the distribution of perlecan and syndecan-4 in the uteri before and after embryo implantation.

Results:  During pre-implantation, perlecan was identified in basement membranes and extracellular spaces of the endometrial stroma. In contrast, expression of syndecan-4 was quite weak. In the peri-implantation period, perlecan remained in the basement membranes, and it was no longer observed in the stroma and it was identified in the embryonic cells. On day 4 of pregnancy, syndecan-4 increased in the fibroblasts of the subepithelial stroma. After implantation, syndecan-4 was pronounced in pre-decidual and mature decidual cells.

Conclusions:  The coordinate balance between the pre- and post-implantation periods suggests a role of these two molecules in the adaptive modification of the uterine microenvironment to receive and implant the embryo.

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