Lectin Receptors for Endometrial H-type 1 Antigen on Goat Conceptuses


Address reprint requests to G. R. Newton, Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446-4079, USA.
E-mail: gary_newton@pvamu.edu


Problem:  The goal of this study was to determine if caprine conceptuses express lectin-like receptors for endometrial H-type 1 (HT1) antigen.

Method of study:  Conceptus tissues were collected during the apposition, adhesion and attachment phases of placentation and evaluated using immunofluorescence microscopy.

Results:  Conceptus staining for the trisaccharide lacto-N-fucopentaose-1 was strong and uniform during apposition of fetal and maternal tissues but changed by day 25 of pregnancy when large aggregates of intense staining were observed. Monoclonal antibodies to galectin-3 did not stain conceptus tissue during the apposition phase but intense punctate staining was observed after day 25. Strong uniform staining for Lewis Y antigen was detected only on day 17 of pregnancy.

Conclusion:  Conceptus tissue expresses potential receptors for endometrial HT1 antigen. Carbohydrate–lectin interactions may facilitate attachment of the apical surfaces of uterine epithelial cells and trophectoderm during the early stages of placentation.