The Effect of Inflammatory Cytokines on Secretion of Macrophage Colony-Stimulating Factor and Monocyte Chemoattractant Protein-1 in Human Granulosa Cells

Authors


Address reprint requests to Yasushi Kawano, Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan.
E-mail: kawayas@med.oita-u.ac.jp

Abstract

Problem:  In order to investigate the role of macrophage colony-stimulating factor (M-CSF) and monocyte chemoattractant protein -1 (MCP-1) in human ovulation, we studied the regulation of M-CSF and MCP-1 in cultured human granulosa cells.

Method of study:  Immortalized granulosa cells (GC1a) were cultured in serum-free medium, and incubated with interleukin (IL)-1α, IL-1 receptor antagonist (ra) and tumor necrosis factor (TNF)-α. The supernatants were collected, and M-CSF and MCP-1 were measured by ELISA.

Results:  The levels of M-CSF and MCP-1 were increased after treatment with IL-1α (1 nm) and TNF-α (1 nm) in a time-dependent manner. The levels of M-CSF and MCP-1 were significantly increased after treatment with IL-1α and TNF-α in a dose-dependent manner. However, the levels of M-CSF and MCP-1 were significantly decreased by treatment with IL-1α (1 nm) and/or increasing concentrations of IL-1 ra.

Conclusions:  Our data indicated that M-CSF and MCP-1 were regulated by IL-1α and TNF-α. It was suggested that M-CSF and MCP-1 may play an important role in human preovulatory processes.

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