The Effect of Inflammatory Cytokines on Secretion of Macrophage Colony-Stimulating Factor and Monocyte Chemoattractant Protein-1 in Human Granulosa Cells
Article first published online: 26 JUL 2004
American Journal of Reproductive Immunology
Volume 52, Issue 2, pages 124–128, August 2004
How to Cite
Kawano, Y., Fukuda, J., Itoh, H., Takai, N., Nasu, K. and Miyakawa, I. (2004), The Effect of Inflammatory Cytokines on Secretion of Macrophage Colony-Stimulating Factor and Monocyte Chemoattractant Protein-1 in Human Granulosa Cells. American Journal of Reproductive Immunology, 52: 124–128. doi: 10.1111/j.1600-0897.2004.00198.x
- Issue published online: 26 JUL 2004
- Article first published online: 26 JUL 2004
- Submitted February 23, 2004; revised May 17, 2004; accepted May 18, 2004.
- Granulosa cell;
- monocyte chemoattractant protein-1;
- macrophage colony-stimulating factor;
Problem: In order to investigate the role of macrophage colony-stimulating factor (M-CSF) and monocyte chemoattractant protein -1 (MCP-1) in human ovulation, we studied the regulation of M-CSF and MCP-1 in cultured human granulosa cells.
Method of study: Immortalized granulosa cells (GC1a) were cultured in serum-free medium, and incubated with interleukin (IL)-1α, IL-1 receptor antagonist (ra) and tumor necrosis factor (TNF)-α. The supernatants were collected, and M-CSF and MCP-1 were measured by ELISA.
Results: The levels of M-CSF and MCP-1 were increased after treatment with IL-1α (1 nm) and TNF-α (1 nm) in a time-dependent manner. The levels of M-CSF and MCP-1 were significantly increased after treatment with IL-1α and TNF-α in a dose-dependent manner. However, the levels of M-CSF and MCP-1 were significantly decreased by treatment with IL-1α (1 nm) and/or increasing concentrations of IL-1 ra.
Conclusions: Our data indicated that M-CSF and MCP-1 were regulated by IL-1α and TNF-α. It was suggested that M-CSF and MCP-1 may play an important role in human preovulatory processes.