The Differential Effect of Genetic Variation on Soluble CD14 Levels in Human Plasma and Milk
Article first published online: 16 SEP 2004
American Journal of Reproductive Immunology
Volume 52, Issue 3, pages 204–211, September 2004
How to Cite
Guerra, S., Carla Lohman, I., LeVan, T. D., Wright, A. L., Martinez, F. D. and Halonen, M. (2004), The Differential Effect of Genetic Variation on Soluble CD14 Levels in Human Plasma and Milk. American Journal of Reproductive Immunology, 52: 204–211. doi: 10.1111/j.1600-0897.2004.00207.x
- Issue published online: 16 SEP 2004
- Article first published online: 16 SEP 2004
- Submitted January 12, 2004; revised June 9, 2004; accepted June 22, 2004.
- CD14 Antigen;
- gene regulation;
- human milk;
- single nucleotide polymorphism
Problem: The protein CD14 is a pattern recognition receptor for bacterial lipopolysaccharide (LPS). Whether genetic variation has the same influence on soluble CD14 (sCD14) levels in human plasma and milk remains to be determined.
Method of study: We measured sCD14 levels in plasma during pregnancy (n = 196) and in milk in the postpartum (n = 152) for women genotyped for the single nucleotide polymorphisms (SNPs) at positions −1619, −550, and −159 from the transcription start site of the CD14 gene.
Results: Plasma- and milk-sCD14 levels differed significantly both by CD14/−1619 and CD14/−550 genotypes and by haplotypes. Most interestingly, sCD14 levels were regulated differentially by the same genetic variants in plasma and milk, with the CD14/−550T allele and the corresponding are ATC haplotype associated with high sCD14 in milk but low sCD14 in plasma. A correlation between sCD14 levels in plasma and milk was absent (r = 0.091, P = NS).
Conclusions: Our findings suggest the existence of cell-specific regulation mechanisms of CD14 gene expression.