Interleukin-1β-induced Expression of IL-6 and Production of Human Chorionic Gonadotropin in Human Trophoblast Cells via Nuclear Factor-κB Activation
Article first published online: 16 SEP 2004
American Journal of Reproductive Immunology
Volume 52, Issue 3, pages 218–223, September 2004
How to Cite
Tsukihara, S., Harada, T., Deura, I., Mitsunari, M., Yoshida, S., Iwabe, T. and Terakawa, N. (2004), Interleukin-1β-induced Expression of IL-6 and Production of Human Chorionic Gonadotropin in Human Trophoblast Cells via Nuclear Factor-κB Activation. American Journal of Reproductive Immunology, 52: 218–223. doi: 10.1111/j.1600-0897.2004.00209.x
- Issue published online: 16 SEP 2004
- Article first published online: 16 SEP 2004
- Submitted May 3, 2004; revised June 4, 2004; accepted July 13, 2004.
- BeWo cells;
- human chorionic gonadotropine;
- nuclear factor-κB
Problem: The cytokine, interleukin-6 (IL-6), stimulates the production of human chorionic gonadotropine (hCG) in chorionic cells. The purpose of this study was to examine the role of nuclear factor-κB (NF-κB) during the induction of IL-6 by IL-1β in human trophoblast cells.
Method of study: Reverse transcription-polymerase chain reaction was used to determine the gene expressions of IL-1, IL-1 receptor (IL-1R), IL-6R and gp130 in a human choriocarcinoma cell line, BeWo. The BeWo cells were cultured for 24 hr with IL-1β (0–10 ng/mL), IL-1β (10 ng/mL) together with IL-1 receptor antagonist (IL-1Ra) or NF-κB inhibitor, N-tocyl-l-phenylalanine chloromethyl ketone (TPCK). The concentrations of IL-6 in culture supernatants were measured by enzyme-linked immunosorbent assay.
Results: The gene expressions of IL-1R, IL-6R and gp130, but not IL-1β, were observed. The concentration of IL-6 was enhanced by IL-1β in a dose-dependent fashion. The addition of IL-1Ra neutralized the effects of IL-1β on IL-6 secretion. IL-1β induced expression of phosphorylated IκB and the activation of NF-κB. The addition of TPCK reduced the IL-1β-induced IL-6 expression. Adding IL-1β increased β-hCG production in a dose-dependent manner, and the addition of TPCK neutralized the effect of IL-1β.
Conclusion: IL-1β may induce the IL-6 expression and hCG production in human BeWo cells via NF-κB.