Increased Numbers of Activated Mast Cells in Endometriosis Lesions Positive for Corticotropin-Releasing Hormone and Urocortin
Article first published online: 18 OCT 2004
American Journal of Reproductive Immunology
Volume 52, Issue 4, pages 267–275, October 2004
How to Cite
Kempuraj, D., Papadopoulou, N., Stanford, E. J., Christodoulou, S., Madhappan, B., Sant, G. R., Solage, K., Adams, T. and Theoharides, T. C. (2004), Increased Numbers of Activated Mast Cells in Endometriosis Lesions Positive for Corticotropin-Releasing Hormone and Urocortin. American Journal of Reproductive Immunology, 52: 267–275. doi: 10.1111/j.1600-0897.2004.00224.x
- Issue published online: 18 OCT 2004
- Article first published online: 18 OCT 2004
- Submitted March 8, 2004; revised August 13, 2004; accepted September 8, 2004.
- Corticotropin-releasing hormone;
- mast cells;
Problem: Mast cells are critical in allergic and inflammatory diseases such as interstitial cystitis, which is often clinically associated with or mistaken as endometriosis. Mast cells had previously been reported to be increased at sites of endometriosis, and tryptase may contribute to the fibrosis and inflammation characterizing endometriosis.
Method of study: This is a pilot study of mast cell numbers and its activation in endometriosis biopsies (n = 10) by immunostaining for mast cell tryptase, corticotropin-releasing hormone (CRH) and urocortin (Ucn).
Results: This is the first report that tryptase positive mast cells were not only increased (64–157 mast cells/mm2) in human endometriosis, but also highly activated (89%) in areas strongly stained positive for CRH/Ucn. Normal endometrium was weakly positive for both CRH/Ucn.
Conclusion: High numbers of activated mast cells are present in endometriosis sites that were strongly positive for CRH/Ucn. CRH and Ucn may activate mast cells and contribute to the fibrosis and inflammation in endometriosis.