Lymphocyte Sub-populations in Gestational Diabetes
Article first published online: 24 JAN 2005
American Journal of Reproductive Immunology
Volume 53, Issue 1, pages 21–29, January 2005
How to Cite
Mahmoud, F., Abul, H., Omu, A. and Haines, D. (2005), Lymphocyte Sub-populations in Gestational Diabetes. American Journal of Reproductive Immunology, 53: 21–29. doi: 10.1111/j.1600-0897.2004.00241.x
- Issue published online: 24 JAN 2005
- Article first published online: 24 JAN 2005
- Submitted May 13, 2004; revised November 17, 2004; accepted November 29, 2004.
- lymphocyte subsets;
Problem: We hypothesize that the normal immunologic responses by the maternal immune system during pregnancy are not as well-regulated in gestational diabetes (GD) patients as in healthy pregnant women.
Method of study: Using two-color flow cytometry we evaluated frequencies of peripheral blood lymphocytes in 20 GD patients being treated with insulin; 43 GD patients treated with dietary therapy but no insulin; 44 women experiencing normal pregnancies; and 48 non-pregnant women.
Results: When compared with healthy pregnant women, both GD cohorts showed higher percentages CD4+CD25+ (P < 0.05), CD4+CD45RO+ (P < 0.05) and CD4+CD29+ (P < 0.01) but lower percentages of CD4+CD45RA+ (P < 0.05). Higher percentages of the activated phenotypes CD8+CD25+ and CD8+HLA-DR+ cells in the diet-treated cohort and CD4+HLA-DR+ cells in insulin-treated GB cohort, were observed compared with healthy pregnant subjects (P < 0.05).
Conclusions: Expanded populations of activated peripheral blood T cells are associated with GD, suggesting that normal maternal immunosuppression is less effective in GD-afflicted women.