Human Endometrial Epithelial Cells (EEC) Constitutively Express More Intercellular Adhesion Molecule (ICAM)-1 than Endometrial Stromal Cells (ESC) in Culture
Article first published online: 9 JUN 2005
American Journal of Reproductive Immunology
Volume 54, Issue 1, pages 5–12, July 2005
How to Cite
Defrère, S., Van Langendonckt, A., Moulin, P., Befahy, P., Gonzalez, D., Martinez-Madrid, B., Dolmans, M.-M. and Donnez, J. (2005), Human Endometrial Epithelial Cells (EEC) Constitutively Express More Intercellular Adhesion Molecule (ICAM)-1 than Endometrial Stromal Cells (ESC) in Culture. American Journal of Reproductive Immunology, 54: 5–12. doi: 10.1111/j.1600-0897.2005.00272.x
- Issue published online: 9 JUN 2005
- Article first published online: 9 JUN 2005
- Submitted January 4, 2005; accepted February 17, 2005.
- epithelial cells;
- intercellular adhesion molecule-1;
- stromal cells
Problem: Intercellular adhesion molecule-1 (ICAM-1) is thought to play an important role in pathophysiological processes in endometrial tissue. The aim of this study was to quantify and compare the expression of ICAM-1 mRNA and protein in cultured endometrial epithelial cells (EEC) versus endometrial stromal cells (ESC).
Method of study: EEC and ESC were isolated from human endometrium and cultured. At confluency, ICAM-1 mRNA was measured by real-time reverse transcriptase-polymerase chain reaction, the membrane-bound form (mICAM-1) by immunocytodensitometry, and the soluble form (sICAM-1) by enzyme-linked immunosorbent assay.
Results: At the transcriptional level, we observed a 1.7-fold increase in ICAM-1 expression in EEC versus ESC. mICAM-1 immunostaining in EEC [cell-relative membrane-bound signal-specific optical density (CR-M-SOD): 0.056 ± 0.05] was stronger (P < 0.05) than in ESC (CR-M-SOD: 0.009 ± 0.07). EEC were found to secrete more (P < 0.01) sICAM-1 ([sICAM-1] = 15.59 ± 2.96 ng/mL) than ESC ([sICAM-1] = 5.14 ± 2.61 ng/mL).
Conclusions: Cultured EEC constitutively express significantly more ICAM-1 mRNA and protein than ESC.