Cytokine Profile of Natural Killer Cells in Early Human Pregnancy
Article first published online: 5 AUG 2005
American Journal of Reproductive Immunology
Volume 54, Issue 1, pages 21–29, July 2005
How to Cite
Higuma-Myojo, S., Sasaki, Y., Miyazaki, S., Sakai, M., Siozaki, A., Miwa, N. and Saito, S. (2005), Cytokine Profile of Natural Killer Cells in Early Human Pregnancy. American Journal of Reproductive Immunology, 54: 21–29. doi: 10.1111/j.1600-0897.2005.00279.x
- Issue published online: 5 AUG 2005
- Article first published online: 5 AUG 2005
- Submitted January 4, 2005; revised March 4, 2005; accepted March 22, 2005.
- CD56bright natural killer cells;
- transforming growth factor-β
Problem: To examine whether the NK1/NK2/NK3/NKr1 paradigm can be adapted in natural killer (NK) cells.
Method of study: Mononuclear cells were isolated from peripheral blood and/or decidua in healthy non-pregnant women (n = 17), early pregnant women (6–12 weeks of gestation, n = 17) and miscarriage cases (6–11 weeks of gestation, n = 10). We investigated the production of transforming growth factor (TGF)-β, interleukin (IL)-4, IL-5, IL-10, IL-13, interferon (IFN)-γ and tumor necrosis factor-α from peripheral blood- and decidual-CD56bright NK cells and -CD56dim NK cells by flow cytometry.
Results: In the peripheral blood of the non-pregnant subjects, the main populations of CD56bright NK cells and CD56dim NK cells were IFN-γ-producing NK1 type cells. Populations of IL-10-producing NKr1 type cells in peripheral blood CD56bright NK cells and CD56dim NK cells in early pregnant women were significantly greater compared with those in non-pregnant women, and these cells population decreased in miscarriage cases. In the early pregnancy decidua, the main populations of CD56bright NK cells and CD56dim NK cells were TGF-β-producing NK3 type cells, and NK1 type cells were rare. NK3 type cells in decidua were significantly decreased in miscarriage cases compared with those in normal pregnant subjects. IL-4-, IL-5- or IL-13-producing NK2 type cells were rare in peripheral blood and decidua.
Conclusion: These data support the NK1/NK2/NK3/NKr1 hypothesis. NKr1 type cells in peripheral blood and NK3 type cells in decidua might play some important roles in the maintenance of pregnancy by regulation of maternal immune function.