Uterine Response to Multiple Inoculations with Arcanobacterium pyogenes and Escherichia coli in Nulliparous Ewes
Version of Record online: 7 OCT 2005
American Journal of Reproductive Immunology
Volume 54, Issue 5, pages 249–261, November 2005
How to Cite
Wulster-Radcliffe, M. C., Seals, R. C. and Lewis, G. S. (2005), Uterine Response to Multiple Inoculations with Arcanobacterium pyogenes and Escherichia coli in Nulliparous Ewes. American Journal of Reproductive Immunology, 54: 249–261. doi: 10.1111/j.1600-0897.2005.00304.x
- Issue online: 7 OCT 2005
- Version of Record online: 7 OCT 2005
- Submitted December 15, 2004; revised June 3, 2005; accepted June 15, 2005.
- Active immunity;
- uterine infection
Uterine infections seem more severe in nulliparous animals. Our objective was to determine whether intrauterine inoculation of nulliparous ewes with Arcanobacterium pyogenes and Escherichia coli would produce an antibody response and reduce the severity of subsequent infections.
Method of study
Nulliparous ewes (n = 9/treatment) received (i) ‘primary intrauterine inoculation’ with phosphate-buffered saline (PBS) and ‘secondary intrauterine inoculation’ with PBS; (ii) primary PBS-secondary 75 × 107 cfu of A. pyogenes and 35 × 107 cfu of E. coli (PBS–Bacteria); (iii) primary bacteria-secondary PBS; or (iv) primary bacteria–secondary bacteria (Bacteria–Bacteria).
Inoculations evoked an antibody response. Postmortem examinations 6 days after the secondary inoculation indicated that PBS-treated ewes did not develop uterine infections, but all bacteria-treated ewes did. Infections were either less severe or closer to resolution in Bacteria–Bacteria than they were in PBS–Bacteria ewes.
Intrauterine inoculation of nulliparous ewes with A. pyogenes and E. coli evokes an antibody response that may help the uterus reduce the severity of subsequent infections.