Neutrophil Function During Pregnancy: Is Nitric Oxide Production Correlated with Superoxide Production?
Article first published online: 19 JAN 2006
American Journal of Reproductive Immunology
Volume 55, Issue 2, pages 99–105, February 2006
How to Cite
Tsukimori, K., Fukushima, K., Komatsu, H. and Nakano, H. (2006), Neutrophil Function During Pregnancy: Is Nitric Oxide Production Correlated with Superoxide Production?. American Journal of Reproductive Immunology, 55: 99–105. doi: 10.1111/j.1600-0897.2005.00324.x
- Issue published online: 19 JAN 2006
- Article first published online: 19 JAN 2006
- Submitted May 2, 2005; revised July 18, 2005; accepted July 22, 2005.
- nitric oxide;
- superoxide anion
Problem Oxygen radical formation by neutrophils during pregnancy is not well studied.
Method of study We studied neutrophil-derived superoxide anion (O) and nitric oxide (NO) values in 75 normal pregnant women, 12 postpartum women, and 10 non-pregnant women. O production was measured by the superoxide dismutase-inhibitable reduction of ferricytochrome c. NO production was measured by accumulation of the stable end product nitrite using a modified Griess reaction method.
Results O production of neutrophils stimulated by chemotactic peptide was significantly enhanced in the early second trimester of pregnancy. l-arginine analogue-inhibitable nitrite production was induced in neutrophils from pregnant women, but not from postpartum and non-pregnant subjects. In third-trimester subjects but not non-pregnant subjects, neutrophils pre-treated with l-arginine analogues enhanced O production compared with untreated neutrophils.
Conclusion These findings indicate that O and NO production by neutrophils during pregnancy were modulated separately, whereas neutrophil-derived NO might function as a regulator of O.