Effects of the Common 677C>T Mutation of the 5,10-Methylenetetrahydrofolate Reductase (MTHFR) Gene on Ovarian Responsiveness to Recombinant Follicle-Stimulating Hormone
Article first published online: 8 MAR 2006
American Journal of Reproductive Immunology
Volume 55, Issue 4, pages 251–258, April 2006
How to Cite
Thaler, C. J., Budiman, H., Ruebsamen, H., Nagel, D. and Lohse, P. (2006), Effects of the Common 677C>T Mutation of the 5,10-Methylenetetrahydrofolate Reductase (MTHFR) Gene on Ovarian Responsiveness to Recombinant Follicle-Stimulating Hormone. American Journal of Reproductive Immunology, 55: 251–258. doi: 10.1111/j.1600-0897.2005.00357.x
- Issue published online: 8 MAR 2006
- Article first published online: 8 MAR 2006
- Submitted August 2, 2005; accepted December 5, 2005.
- Controlled ovarian hyperstimulation;
- follicle-stimulating hormone;
- 5,10-methylene tetrahydrofolate reductase;
- ovarian reserve;
- ovarian responsiveness
Problem The common 677C>T mutation of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene is less prevalent in mothers of spontaneously conceived dichorionic twins than in those with singleton pregnancies. (Hasbargen U, Lohse P, Thaler CJ: Hum Reprod 2000; 15:2659–2662). As polyovulation is a pre-requisite for dichorionic pregnancies, we investigated whether the T allele is associated with reduced ovarian responsiveness to follicle-stimulating hormone (FSH).
Materials and methods We studied 105 infertility patients undergoing a total of 269 A.R.T cycles stimulated with recombinant (r)-FSH. The MTHFR 677C>T genotype was analyzed by digestion of polymerase chain reaction–amplified DNA fragments.
Results Homozygous 677C patients older than 35, required significantly (P <0.02) less r-FSH and produced significantly more oocytes (P < 0.04) and higher maximal serum estradiol concentrations (P < 0.002) than heterozygous or homozygous carriers of the 677T allele.
Conclusion Our results suggest that, in patients of advanced reproductive age, the MTHFR 677C>T mutation affects ovarian responsiveness.