Increased Interleukin-6 Messenger RNA Expression in Macrophage-Cocultured Endometrial Stromal Cells in Adenomyosis
Article first published online: 1 FEB 2006
American Journal of Reproductive Immunology
Volume 55, Issue 3, pages 181–187, March 2006
How to Cite
Yang, J.-H., Wu, M.-Y., Chang, D.-Y., Chang, C.-H., Yang, Y.-S. and Ho, H.-N. (2006), Increased Interleukin-6 Messenger RNA Expression in Macrophage-Cocultured Endometrial Stromal Cells in Adenomyosis. American Journal of Reproductive Immunology, 55: 181–187. doi: 10.1111/j.1600-0897.2005.00363.x
- Issue published online: 1 FEB 2006
- Article first published online: 1 FEB 2006
- Submitted August 1, 2005; accepted October 21, 2005.
- endometrial stromal cell;
To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis.
Method of study
Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr.
Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis.
IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.