Possible Role of Natural Immune Response against Altered Fibroblasts in the Development of Post-Operative Adhesions

Authors

  • Zeynep Alpay,

    1. Hutzel Hospital, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Wayne State University, Detroit, MI, USA
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  • Melike S. Özgönenel,

    1. Children's Hospital of Michigan, The Carman Ann Adams Department of Pediatrics, Division of Hematology/Oncology, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA
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  • Süreyya Savaşan,

    1. Children's Hospital of Michigan, The Carman Ann Adams Department of Pediatrics, Division of Hematology/Oncology, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA
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  • Steven Buck,

    1. Children's Hospital of Michigan, The Carman Ann Adams Department of Pediatrics, Division of Hematology/Oncology, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA
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  • Ghassan M. Saed,

    1. Hutzel Hospital, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Wayne State University, Detroit, MI, USA
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  • Michael P. Diamond

    1. Hutzel Hospital, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Wayne State University, Detroit, MI, USA
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Michael P. Diamond, 3750 Woodward Ave., Suite 200-D, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Detroit, MI 48201, USA.
E-mail: mdiamond@med.wayne.edu

Abstract

Problem

Post-operative adhesion tissue fibroblasts (ATF) differ from normal peritoneal fibroblasts (NPF). Natural immune response participates in the elimination of altered cells. In this study, we investigated NPF and ATF expression patterns of immune response-related markers, and lymphokine-activated killer (LAK) cell-mediated fibroblast elimination in vitro.

Method of study

Primary cell cultures of both NPF and ATF obtained from the same four patients were used in the experiments. The expression of CD54, CD40 and CD120b, and allogeneic LAK cell-mediated ATF and NPF elimination were studied by flow cytometry.

Results

Average expression of CD54 in ATF was greater by 12.3-fold compared with NPF (P = 0.021), with ratios of 2.4 and 1.9-fold for CD40 (P < 0.001) and CD120b (P = 0.013), respectively. Average LAK cell-mediated fibroblast killing was 1.8 ± 0.8-fold greater in ATF over NPF (P = 0.008). Furthermore, LAK cell-mediated fibroblast elimination correlated significantly with the increased CD40, CD54 and CD120b expression (R > 0.956; P < 0.05 for each).

Conclusions

These results demonstrate that ATF are more susceptible to lymphocyte-mediated elimination than NPF and the development of adhesions despite this could be explained by either impaired or overwhelmed autologous natural immune response against reactive fibroblasts.

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