Increased IL-18 Levels in Seminal Plasma of Infertile Men with Genital Tract Infections
Article first published online: 3 MAY 2006
American Journal of Reproductive Immunology
Volume 55, Issue 6, pages 428–433, June 2006
How to Cite
Matalliotakis, I. M., Cakmak, H., Fragouli, Y., Kourtis, A., Arici, A. and Huszar, G. (2006), Increased IL-18 Levels in Seminal Plasma of Infertile Men with Genital Tract Infections. American Journal of Reproductive Immunology, 55: 428–433. doi: 10.1111/j.1600-0897.2006.00380.x
- Issue published online: 3 MAY 2006
- Article first published online: 3 MAY 2006
- Submitted December 14, 2005; accepted March 1, 2006.
- seminal plasma;
- urogenital infection
Interleukin (IL)-18 is a novel cytokine, previously known as interferon (IFN)-γ inducing factor. We evaluated the levels of IL-18 and IFN-γ in seminal plasma (SP) of fertile and infertile men.
Method of study
Semen samples were obtained by masturbation from 80 men, and were examined for the levels of IL-18 and IFN-γ by enzyme-linked immunosorbent assay. Seven groups were included: (i) fertile men (n = 18), (i) infertile men with genital tract infections (n = 17), (iii) with varicocele (n = 15), (iv) with Klinefelter syndrome (n = 6), (v) with cryptorchidism (n = 7), (vi) with mumps orchitis (n = 7), and (vii) with idiopathic testicular lesions (n = 10).
Mean levels of IL-18 were higher in SP from infertile men with genital tract infections compared with SP from other groups except Klinefelter syndrome (P < 0.05). However, no significant differences could be detected for IFN-γ. A significant positive correlations was found between IL-18 and IFN-γ in total patient population (P < 0.001). Moreover, a negative correlation was observed between IL-18 and sperm concentrations, and motility (P < 0.01 and <0.03, respectively). Furthermore, there was a positive and statistically significant association between IL-18 and IFN-γ levels in SP of infertile men with genital tract infections (P < 0.0001). However, there was no relationship between IL-18 and IFN-γ, and semen parameters in the same group.
SP IL-18 levels were increased in men with urogenital infections. Thus, the elevated expression of IL-18 in SP may be used as a diagnostic marker in the male genital tract infections.