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Keywords:

  • cytokine;
  • embryo;
  • growth factor;
  • placenta;
  • pregnancy

Problem

Uterine-derived factors are required for optimal conceptus development and secretion of the maternal recognition of pregnancy factor, interferon-τ (IFN-τ). Identifying these factors may lead to the development of schemes for increasing pregnancy success in cattle.

Method of Study

The objectives were to examine the effects of granulocyte-macrophage colony-stimulating-factor (GM-CSF) on trophectoderm proliferation rates and IFN-τ production, and verify the appropriateness of using an in vitro model of bovine trophectoderm (CT-1 cell).

Results

Rate of [3H]-thymidine incorporation into DNA was increased by supplementation of CT-1 medium with 10 or 100 ng/mL porcine (po) GM-CSF. GM-CSF supplementation to CT-1 medium also increased IFN-τ secretion. When results were normalized to account for number of CT-1 cells, 10 and 100 ng/mL poGM-CSF increased antiviral activity and IFN-τ concentrations (using an IFN-τ-specific enzyme-linked immunosorbent assay) in CT-1 conditioned medium compared with controls.

Conclusions

These findings indicate that GM-CSF increases proliferation and IFN-τ production in bovine trophectoderm.