Granulocyte-Macrophage Colony-Stimulating-Factor Increases Interferon-τ Protein Secretion in Bovine Trophectoderm Cells
Article first published online: 17 MAY 2006
American Journal of Reproductive Immunology
Volume 56, Issue 1, pages 63–67, July 2006
How to Cite
Michael, D. D., Wagner, S. K., Ocón, O. M., Talbot, N. C., Rooke, J. A. and Ealy, A. D. (2006), Granulocyte-Macrophage Colony-Stimulating-Factor Increases Interferon-τ Protein Secretion in Bovine Trophectoderm Cells. American Journal of Reproductive Immunology, 56: 63–67. doi: 10.1111/j.1600-0897.2006.00390.x
- Issue published online: 17 MAY 2006
- Article first published online: 17 MAY 2006
- Submitted February 3, 2006; revised March 21, 2006; accepted March 27, 2006
- growth factor;
Uterine-derived factors are required for optimal conceptus development and secretion of the maternal recognition of pregnancy factor, interferon-τ (IFN-τ). Identifying these factors may lead to the development of schemes for increasing pregnancy success in cattle.
Method of Study
The objectives were to examine the effects of granulocyte-macrophage colony-stimulating-factor (GM-CSF) on trophectoderm proliferation rates and IFN-τ production, and verify the appropriateness of using an in vitro model of bovine trophectoderm (CT-1 cell).
Rate of [3H]-thymidine incorporation into DNA was increased by supplementation of CT-1 medium with 10 or 100 ng/mL porcine (po) GM-CSF. GM-CSF supplementation to CT-1 medium also increased IFN-τ secretion. When results were normalized to account for number of CT-1 cells, 10 and 100 ng/mL poGM-CSF increased antiviral activity and IFN-τ concentrations (using an IFN-τ-specific enzyme-linked immunosorbent assay) in CT-1 conditioned medium compared with controls.
These findings indicate that GM-CSF increases proliferation and IFN-τ production in bovine trophectoderm.