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Granulocyte-Macrophage Colony-Stimulating-Factor Increases Interferon-τ Protein Secretion in Bovine Trophectoderm Cells

Authors


Dr Alan D. Ealy, Department of Animal Sciences, University of Florida, PO Box 110910, Gainesville, FL 32611-0910, USA.
E-mail: ealy@ufl.edu

Present addresses: Donna D. Michael, Department of Physiology, Dartmouth Medical School, Borwell Building 744 W, 1 Medical Center Drive, Lebanon, NH 03756, USA.

Sara K. Wagner, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.

Olga M. Ocón, Department of Poultry Science, Pennsylvania State University, 211 Henning Building, University Park, PA 16811, USA.

Abstract

Problem

Uterine-derived factors are required for optimal conceptus development and secretion of the maternal recognition of pregnancy factor, interferon-τ (IFN-τ). Identifying these factors may lead to the development of schemes for increasing pregnancy success in cattle.

Method of Study

The objectives were to examine the effects of granulocyte-macrophage colony-stimulating-factor (GM-CSF) on trophectoderm proliferation rates and IFN-τ production, and verify the appropriateness of using an in vitro model of bovine trophectoderm (CT-1 cell).

Results

Rate of [3H]-thymidine incorporation into DNA was increased by supplementation of CT-1 medium with 10 or 100 ng/mL porcine (po) GM-CSF. GM-CSF supplementation to CT-1 medium also increased IFN-τ secretion. When results were normalized to account for number of CT-1 cells, 10 and 100 ng/mL poGM-CSF increased antiviral activity and IFN-τ concentrations (using an IFN-τ-specific enzyme-linked immunosorbent assay) in CT-1 conditioned medium compared with controls.

Conclusions

These findings indicate that GM-CSF increases proliferation and IFN-τ production in bovine trophectoderm.

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