Downregulation of Human Endometrial IL-18 by Exogenous Ovarian Steroids

Authors

  • Nathalie Lédée,

    1. Université Versailles- St Quentin en Yvelines, Centre Hospitalier de Poissy- St Germain en Laye, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Poissy, France;
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  • Sylvie Dubanchet,

    1. Unité 782 INSERM - UMR-S 782, Endocrinologie et Génétique de la Reproduction et du Développement, Clamart, France
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  • Raoul Lombroso,

    1. Université Versailles- St Quentin en Yvelines, Centre Hospitalier de Poissy- St Germain en Laye, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Poissy, France;
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  • Yves Ville,

    1. Université Versailles- St Quentin en Yvelines, Centre Hospitalier de Poissy- St Germain en Laye, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Poissy, France;
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  • Gérard Chaouat

    1. Unité 782 INSERM - UMR-S 782, Endocrinologie et Génétique de la Reproduction et du Développement, Clamart, France
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N. Lédée, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, Centre Hospitalier de Poissy- St Germain en Laye, 10 rue du champ Gaillard, 78300, Poissy, France.
E-mail: ledeenathalie@aol.com

Abstract

Problem

To evaluate the influence of ovarian steroids on IL-18, IL-15 and angiopoietin-2 mRNA expression in the endometrium in the mid luteal phase.

Method of study

We quantified IL-18/GAPDH, IL-18 BP/GAPDH, IL-15/GAPDH and angiopoietin-2/GAPDH in the endometrium by quantitative polymerase chain reaction on day 21 of the cycle. We first compared cytokines expression over two natural cycles (n = 15) then between natural and oestrogen–progestin replacement treatment (n = 18).

Results

Endometrial IL-18, IL-18 BP, IL-15 and angiopoietin-2 mRNA expression did not change over two natural cycles. Addition of exogenous hormones significantly decreased IL-18 and IL-18 BP mRNA expression but not influence IL-15 or angiopoietin-2 ratios. This was also observed with immunohistochemistry.

Conclusion

Exogenous oestro-progestative hormones influence endometrial IL-18 system expression involved in angiogenesis and in the uterine natural killer (uNK) cell activation pathway during the implantation process.

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