Human Leukocyte Antigen F Protein is Expressed in the Extra-Villous Trophoblasts but Not on the Cell Surface of them
Article first published online: 2 AUG 2006
American Journal of Reproductive Immunology
Volume 56, Issue 3, pages 172–177, September 2006
How to Cite
Nagamatsu, T., Fujii, T., Matsumoto, J., Yamashita, T., Kozuma, S. and Taketani, Y. (2006), Human Leukocyte Antigen F Protein is Expressed in the Extra-Villous Trophoblasts but Not on the Cell Surface of them. American Journal of Reproductive Immunology, 56: 172–177. doi: 10.1111/j.1600-0897.2006.00414.x
- Issue published online: 2 AUG 2006
- Article first published online: 2 AUG 2006
- Submitted March 31, 2006; accepted May 15, 2006.
- extra-villous trophoblast;
- human leukocyte antigen-F;
Problem Human leukocyte antigen (HLA)-F together with other class 1b HLAs may play a pivotal role in immunological interaction at feto-maternal interface. However, it is not elucidated whether HLA-F is expressed on cell surface of extra-villous trophoblasts (EVTs).
Method of study The localization of HLA-F in placenta of each trimester was investigated by immunohistochemistry. The expression modality of HLA-F molecule was compared with that of HLA-G in cultured EVTs using flowcytometry.
Results and conclusion In immunohistochemical study, remarkable staining for HLA-F was confirmed in EVTs throughout gestation. HLA-F expression was restricted to be intracellular in cultured EVTs. HLA-F expression was increased with time in culture, which was accompanied by the increase of cell surface HLA-G. Consequently, HLA-F lacking in cell surface expression on EVTs may not contribute to the direct cell-to-cell immune reaction but might work supportively modifying the function of HLA-G.