Butyrylcholinesterase Activity in Gestational Diabetes: Correlation with Lymphocyte Subpopulations in Peripheral Blood
Article first published online: 23 JUN 2006
American Journal of Reproductive Immunology
Volume 56, Issue 3, pages 185–192, September 2006
How to Cite
Mahmoud, F. F., Haines, D. D., Abul, H. T., Omu, A. E. and Abu-Donia, M. B. (2006), Butyrylcholinesterase Activity in Gestational Diabetes: Correlation with Lymphocyte Subpopulations in Peripheral Blood. American Journal of Reproductive Immunology, 56: 185–192. doi: 10.1111/j.1600-0897.2006.00416.x
- Issue published online: 2 AUG 2006
- Article first published online: 23 JUN 2006
- Submitted February 13, 2006; accepted May 19, 2006.
Problem Inefficient clearance of pregnancy-threatening toxins may contribute to gestational diabetes (GD) and Type II diabetes mellitus (DM) through mechanisms involving immune dysregulation.
Method of Study Peripheral venous blood from pregnant Kuwaiti women in third trimester, including 15 GD and 17 DM patients, 14 healthy pregnant (HP) and eight non-pregnant subjects, was analyzed by two-color flow cytometery for number and percentage representation of T lymphocytes. Buterylcholinesterase (BuChE) activity was measured using buterylthiocholine iodide and spectrophotometry.
Results Relative to HP, GD patients exhibited higher ratios of activated and memory phenotypes, including CD4+ CD25+ (P < 0.01), CD4+ HLA-DR (P < 0.05) and CD4+ CD45RO+ (P < 0.05) cells. Serum BuChE activity exhibited positive correlation within the HP cohort with CD4+ CD25+ (P < 0.05), but not in GD and DM cohorts.
Conclusions Positive correlation between BuChE and a (presumptive) ‘regulatory’ T-cell phenotype in HP, but not GD or DM may indicate existence of protective detoxification mechanisms against oxidative stress in normal pregnancies.