Adoptive Transfer of Paternal Antigen-Hyporesponsive T Cells Facilitates a Th2 Bias in Peripheral Lymphocytes and at Materno-Fetal Interface in Murine Abortion-prone Matings
Article first published online: 25 AUG 2006
American Journal of Reproductive Immunology
Volume 56, Issue 4, pages 258–266, October 2006
How to Cite
Jin, L.-P., Zhou, Y.-H., Zhu, X.-Y., Wang, M.-Y. and Li, D.-J. (2006), Adoptive Transfer of Paternal Antigen-Hyporesponsive T Cells Facilitates a Th2 Bias in Peripheral Lymphocytes and at Materno-Fetal Interface in Murine Abortion-prone Matings. American Journal of Reproductive Immunology, 56: 258–266. doi: 10.1111/j.1600-0897.2006.00425.x
- Issue published online: 25 AUG 2006
- Article first published online: 25 AUG 2006
- Submitted December 6, 2005; accepted June 19, 2006.
- Adoptive transfer;
- paternal antigen-hyporesponsive T cells;
- pregnant outcome;
- recurrent spontaneous abortion;
- Th1/Th2 cytokines
Problem To investigate the Th1/Th2 cytokine changes in abortion-prone recipient mice adoptively transferred by the paternal antigen-hyporesponsive T cells.
Method of study The paternal antigen-hyporesponsive T cells were generated by the anti-B7 monoclonal antibody (mAb) treatment and adoptively transferred into pregnant CBA/J mice of abortion-prone matings on day 4 of gestation. The intracellular expressions of Th1 cell-derived cytokine, tumor necrosis factor-α, γ-interferon and interleukin-2 (IL-2) and Th2 cell-derived cytokine, IL-4 and IL-10 in the maternal spleen were analyzed by flow cytometry, and secretions of the Th1 and Th2 cytokines in supernatant of the feto-placental unit culture were analyzed by an enzyme-linked immunosorbent assay.
Results Our findings showed the increased secretion of Th1 cytokines and the decreased secretion of Th2 cytokines in abortion-prone matings. Treatment with anti-B7 mAbs on day 4 of gestation enhanced Th2 and reduced Th1 cytokine production in abortion-prone matings. Similarly, adoptive transfer of paternal antigen-hyporesponsive T cells induced maternal tolerance to the fetus and displayed a Th2 bias both in the peripheral lymphocytes and at the materno-fetal interface of the abortion-prone matings.
Conclusions These findings indicate that the Th2 cytokine bias and an increase in fetal viability induced by the anti-B7 mAb treatment can be transferred to other pregnant mice of the abortion-prone matings.