Both senior researchers contributed equally to this work.
Induction of Maternal Tolerance to Fetal Alloantigens by RANTES Production
Article first published online: 25 SEP 2006
American Journal of Reproductive Immunology
Volume 56, Issue 5-6, pages 302–311, November/December 2006
How to Cite
Ramhorst, R., Patel, R., Corigliano, A., Etchepareborda, J. J., Fainboim, L. and Schust, D. (2006), Induction of Maternal Tolerance to Fetal Alloantigens by RANTES Production. American Journal of Reproductive Immunology, 56: 302–311. doi: 10.1111/j.1600-0897.2006.00430.x
- Issue published online: 25 SEP 2006
- Article first published online: 25 SEP 2006
- Submitted August 2, 2004; accepted October 7, 2004.
- Maternal tolerance;
- recurrent pregnancy loss
Problem Previous studies have demonstrated a requirement for RANTES (regulated on activated normal T-cell expressed, and secreted) at immune privileged sites; we have investigated the role of RANTES in the induction of maternal–fetal tolerance.
Method of study Endometrial and peripheral T lymphocytes were obtained from women with recurrent pregnancy losses (RPLs) and fertile women. RANTES modulation by progesterone or paternal alloantigens was measured by enzyme-linked immunosorbent assay or flow cytometry analysis.
Results Progesterone significantly increased intracellular RANTES expression in CD4+ and CD8+ endometrial T cells. Moreover, alloreactive lymphocytes from RPL patients produced lower RANTES levels when compared with those from fertile women. At the local level, treatment with recombinant RANTES induced a decrease in CCR5 and CXCR4 messenger RNA that correlated with an increase in T-bet expression. RPL patients and normally fertile women express RANTES similarly, but differ in their patterns of RANTES receptor expression.
Conclusion RANTES may be implicated in the local induction of a Th1-type response necessary for successful implantation. Altered response to RANTES stimulation among some RPL patients may be responsible for poor pregnancy outcomes.